Just wondering whats the current status regarding nitric oxide and rosacea. Is it still considered a cause or issue or is it just another dr. nase theory. Read the study below that lutein helps reduce nitric oxide levels. Lutein apparentely is contained in things like spinach. However if you google spinach its says that it increase lutein levels, so maybe a lutein supplement would be more beneficial. Not sure if i have read study correctely due to the terminology used, ie would lutein supplements alone decrease nitric oxide. Also how much lutein if beneficail would you have to take?

Dietary lutein modulates inducible nitric oxide synthase (iNOS) gene and protein expression in mouse macrophage cells (RAW 264.7)
Mohamed M. Rafi, Yassaman ShafaieArticle first published online: 5 MAR 2007

DOI: 10.1002/mnfr.200600170

Copyright 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Molecular Nutrition & Food Research
Volume 51, Issue 3, pages 333340, March 2007
Additional Information(Show All)
How to CiteAuthor InformationPublication HistoryFunding Information
How to Cite
Rafi, M. M. and Shafaie, Y. (2007), Dietary lutein modulates inducible nitric oxide synthase (iNOS) gene and protein expression in mouse macrophage cells (RAW 264.7). Molecular Nutrition & Food Research, 51: 333340. doi: 10.1002/mnfr.200600170

Author Information
Department of Food Science, Cook College, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA. Fax: +1-732-932-6776
Email: Mohamed M. Rafi (rafi@rci.rutgers.edu)

Publication History
Issue published online: 5 MAR 2007
Article first published online: 5 MAR 2007
Manuscript Revised: 29 NOV 2006
Manuscript Received: 8 SEP 2006
Funded by
New Jersey Agricultural Experiment Stations Hatch Project
Center for Advanced Food Technolo

Keywords:Anti-inflammatory;iNOS;Lutein;Macrophage;Nitric oxide
Lutein is an oxycarotenoid primarily found in dark-green leafy vegetables such as spinach and kale. Other dietary sources which contain moderate amounts of lutein include corn, egg yolks, and fruits like oranges and kiwi. Although a number of in vivo studies have demonstrated the anti-inflammatory effect of lutein, its in vitro anti-inflammatory molecular mechanism of action is unknown. In this study, we have investigated the in vitro anti-inflammatory effect of lutein using LPS-stimulated mouse macrophage cell line (RAW 264.7). The inhibition of LPS-stimulated nitric oxide (NO) was measured and the expression of inducible NO synthase (iNOS) was assessed at the mRNA and protein levels in mouse macrophage cells after treatment with lutein. Lutein decreased the LPS-induced NO production by 50% compared to LPS alone. Real-time PCR analysis showed a 1.9-fold reduction in iNOS expression at the mRNA level. Western blotting revealed that lutein decreased LPS-induced iNOS expression at the protein level by 72.5%. The results of this study suggest the anti-inflammatory properties of lutein demonstrated by the decrease in the expression of iNOS at the mRNA and protein levels in RAW 264.7 mouse macrophage cells.